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scf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec scf
    Scf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+scf/MACS+GMP+Recombinant+Human+SCF/us12698315-701-50-51
    Average 93 stars, based on 4 article reviews
    scf - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Double-negative T cells utilize a TNFα–JAK1–ICAM-1 cytotoxic axis against acute myeloid leukemia
    Article Snippet: .. OCI-AML2 and MV4-11 were cultured in IMDM, OCI-AML3 in alpha-MEM, KG-1a and NALM-6 in RPMI-1640, and TEX in IMDM with IL-3 (R&D Systems, 2 ηg/mL) and human SCF (Miltenyi Biotec, 20 ηg/mL). ..

    Article Title: Treatment of type 1 diabetes and autoimmune diseases or disorders
    Article Snippet: 1×105 of isolated human CD34+ HSPCs (purity 99%) were cultured in 200 μl of StemSpan SFEM II media (SEMCELL Technologies Inc., Cambridge, MA, USA), and each compound in the Prostaglandin Screening Library II (Cayman Chemicals, Ann Arbor, MI), was added individually at day 0 and at day 1 at a concentration of 10 μM as previously reported by the inventors and others (9, 21). .. In another assay, isolated CD34+ cells from freshly isolated human PBMCs or from cryopreserved PBMCs, and processed as described earlier, were cultured in the presence of a cocktail of cytokines containing: 10 μg/ml heparin (SEMCELL Technologies Inc., Cambridge, MA, USA), 10 ng/ml human SCF (Miltenyi Biotec, San Diego, CA), 20 ng/ml human TPO (Miltenyi Biotec, San Diego, CA), 10 ng/ml human FGF-1 (Miltenyi Biotec, San Diego, CA), 100 ng/ml IGFBP2 (R&D Systems, Inc., Minneapolis, MN), and 500 ng/ml Angptl3 (R&D Systems, Inc., Minneapolis, MN). .. PGE2 (PromoKine, PromoCell Gmbh, Germany) was added by pulsing the culture at 0, 24h, 72h and 6 days with 2 μl of diluted PGE2 (10 μM).

    Recombinant:

    Article Title: Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia
    Article Snippet: Peptide, recombinant protein , human BMP-4 , GIBCO , Cat. #: PHC9534 , . .. Peptide, recombinant protein , human SCF , Miltenyi Biotec , Cat. #: 130096692 , . .. Peptide, recombinant protein , human VEGF , GIBCO , Cat. #: PHC9394 , .

    Article Title: microRNA-132 attenuates inflammation in induced pluripotent stem cell-derived microglia from Alzheimer’s disease patients
    Article Snippet: In brief, iPSCs were thawed in a matrigel-coated 6-well plate (Corning Matrigel hESC-Qualified, Cat#354277) using mTeSR TM 1 medium (Cat#85850, Stemcell), with the addition of 10 μM Y-27632 (ROCK inhibitor, Cat#72304, Stemcell) for the first 24 h. Once the cells reached confluence, they were transferred to an AggreWellTM 800 24-well plate (Cat#34815, Stemcell) to form Embryoid Bodies (EBs) at a density of 4 × 10 6 cells per well. .. The EBs were fed daily by changing 75% of the medium with mTeSR1 containing 50 ng/mL Human BMP-4 Recombinant Protein (Cat#PHC9531, Thermo Fisher Scientific), 50 ng/mL Human VEGF-165 Recombinant Protein (Cat#100–20, Peprotech), and 20 ng/mL Human SCF (Cat#130-096-693, Miltenyi Biotec). .. After four days, EBs were transferred to 6-well cell culture plates at a density of 10 to 20 EBs per well, containing X-VIVO15 (Cat#BE02-060F, Lonza) supplemented with 2 mM GlutaMAXTM(Cat#35050038, Life Technologies), 50 μM β-mercaptoethanol (Cat#31350010, Thermo Fisher Scientific), 50 U/ml penicillin/streptomycin (Cat#P4333-100 mL, Sigma-Aldrich), 25 ng/mL Human IL-3 Recombinant Protein (Cat#PHC0035, Thermo Fisher Scientific), and 100 ng/mL Human M-CSF Recombinant Protein (Cat#PHC9501, Thermo Fisher Scientific).

    Isolation:

    Article Title: Treatment of type 1 diabetes and autoimmune diseases or disorders
    Article Snippet: 1×105 of isolated human CD34+ HSPCs (purity 99%) were cultured in 200 μl of StemSpan SFEM II media (SEMCELL Technologies Inc., Cambridge, MA, USA), and each compound in the Prostaglandin Screening Library II (Cayman Chemicals, Ann Arbor, MI), was added individually at day 0 and at day 1 at a concentration of 10 μM as previously reported by the inventors and others (9, 21). .. In another assay, isolated CD34+ cells from freshly isolated human PBMCs or from cryopreserved PBMCs, and processed as described earlier, were cultured in the presence of a cocktail of cytokines containing: 10 μg/ml heparin (SEMCELL Technologies Inc., Cambridge, MA, USA), 10 ng/ml human SCF (Miltenyi Biotec, San Diego, CA), 20 ng/ml human TPO (Miltenyi Biotec, San Diego, CA), 10 ng/ml human FGF-1 (Miltenyi Biotec, San Diego, CA), 100 ng/ml IGFBP2 (R&D Systems, Inc., Minneapolis, MN), and 500 ng/ml Angptl3 (R&D Systems, Inc., Minneapolis, MN). .. PGE2 (PromoKine, PromoCell Gmbh, Germany) was added by pulsing the culture at 0, 24h, 72h and 6 days with 2 μl of diluted PGE2 (10 μM).



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